Technological projects portfolio

Super-resolution microscopy down to 15nm

Investment: €332k

Scope: Healthcare

Scientific field(s): Biology and medicinal chemistry / Physics-Chemistry and Optics

Institution(s): Université Paris-Saclay - CNRS

Development: Start-up in progress/completed

#Microscopy #SuperResolution #ScientificInstruments


Today, it is possible to observe biological entities through a microscope, down to the scale of one molecule, in order to understand the mechanisms involved and find relevant therapeutic solutions.

Recent developments based on the work awarded the 2014 Nobel Prize in Chemistry, have made it possible to improve the resolution of microscopes. However, performance is not equivalent in all three directions, and obtaining nanometric axial resolution remains a major issue.

The NANOLIGHT technology offers a solution to go beyond the axial limitation, improve lateral resolution and therefore dramatically increase the resolution power of microscopes


The NANOLIGHT technology transforms all standard optical microscopes into 3D nanoscopes, offering unmatched visualization of cellular mechanisms that could not be observed up to now. The technology improves 3D isotropic localization from the slide and the axial resolution.

Its ability to be integrated in an optical module for microscopes, the reconstruction and analysis software, as well as the effective chemical buffer make it easy to use.

By improving the equipment already present in laboratories, the technology limits the imposed obsolescence of scientific instruments that weighs on the investment budgets of research institutes.


This technology can be used in fields of academic research and in the pharmaceuticals, agri-food or cosmetics industries.

It meets two major needs of the scientific community. On the one hand, it makes it possible to simultaneously observe internalization and motility events specific to the membrane, in parallel with intracellular events. And on the other hand, it allows for absolute localization of fluorescent molecules with quasi-isotropic nanometric precision when monitoring unique molecules.